NMRFAM Pulse Program Downloads (Agilent / Bruker)


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Jump to: [Agilent spectrometers] [Bruker spectrometers]
Link to [NMRFAM Software Downloads]
Link to [NMRFAM Script Downloads]
Link to [NMRFAM Official Web Site]

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Agilent spectrometers [Installation Guide]

Pulse programs
Descriptions

Pulse programs for PROTEIN samples
Regular PROTEINS


sensitivity-enhanced 15N-HSQC

fast 15N-HSQC with TROSY option

estimate effective correlation time of a protein by measuring the relaxation rate of 15NH amide alfa and beta states

Nz exchange experiment based on the fast 15N-HSQC pulse sequence

Long-range HNCO experiment to detect NH-OC H-bonds in proteins

A set of 13C-filtered 15N-HSQC experiments developed at NMRFAM for the studying of multi-protein complexes (available soon)
Large PROTEINS


2D N,H 15N-TROSY experiment with Nietlispach modification to suppress unwanted anti-TROSY components without any extra loss in sensitivity

3D C,C,H 13C-HMQC NOESY 13C-HMQC experiment for selectively labeled proteins
NUCLEIC ACIDS
(available soon)



Bruker spectrometers [Installation Guide]

Pulse programs
Descriptions

In the download zip file, there are 6 pulse sequence files, 3 for non-constant time (HSQC1, HSQC2 and HSQC3) and 3 for constant time (HSQC1, HSQC2 and HSQC3) and an instruction file on how to set up the experiments. (Kaifeng Hu, William M. Westler, and John L. Markley, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0), (2011) J. Am. Chem. Soc., 133(6), 1662-5.)
PROTEINS


Pulse programs for PROTEIN samples (Legacy)
NUCLEIC ACIDS


Pulse programs for NUCLEIC ACID samples

2D NOESY with presat

2D NOESY with 1-1 echo solvent suppression

2D NOESY with 3-9-19 watergate solvent suppression

2D NOESY with Watergate 5 solvent suppression

1H-15N 2D HMQC

1H-13C 2D HMQC

2D 1H Total Correlation

2D 1H-1H Correlation

F2 filtered 2D NOESY

F1/F2 filtered 2D NOESY

F1 filtered/F2 edited 2D NOESY

ARTSY HSQC to measure aromatic C-H RDCs

ARTSY HSQC to measure imino N-H RDCs

ARTSY HSQC to measure C5-H5 RDCs
Adjunct .incl files


Required include file for general Pulse Program functioning on Bruker spectrometers
Useful macros for TopSpin/XWINNMR


unified scale referencing for X-nucleus indirect dimension of n-dimensional spectra


Jump to: [Agilent spectrometers] [Bruker spectrometers] [Top]
Link to [NMRFAM Software Downloads]
Link to [NMRFAM Script Downloads]
Link to [NMRFAM Official Web Site]

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If there is any questions and comments, contact Dr. Woonghee Lee.


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