NMRFAM Pulse Program Downloads (Agilent / Bruker)

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Agilent spectrometers [Installation Guide]

Pulse programs
 Descriptions

Pulse programs for PROTEIN samples
Regular PROTEINS

sensitivity-enhanced 15N-HSQC

fast 15N-HSQC with TROSY option

estimate effective correlation time of a protein by measuring the relaxation rate of 15NH amide alfa and beta states

Nz exchange experiment based on the fast 15N-HSQC pulse sequence

Long-range HNCO experiment to detect NH-OC H-bonds in proteins

A set of 13C-filtered 15N-HSQC experiments developed at NMRFAM for the studying of multi-protein complexes (available soon)
Large PROTEINS

2D N,H 15N-TROSY experiment with Nietlispach modification to suppress unwanted anti-TROSY components without any extra loss in sensitivity

3D C,C,H 13C-HMQC NOESY 13C-HMQC experiment for selectively labeled proteins
NUCLEIC ACIDS
 (available soon)


Bruker spectrometers [Installation Guide]

Pulse programs
 Descriptions

In the download zip file, there are 6 pulse sequence files, 3 for non-constant time (HSQC1, HSQC2 and HSQC3) and 3 for constant time (HSQC1, HSQC2 and HSQC3) and an instruction file on how to set up the experiments. (Kaifeng Hu, William M. Westler, and John L. Markley, Simultaneous quantification and identification of individual chemicals in metabolite mixtures by two-dimensional extrapolated time-zero 1H-13C HSQC (HSQC0), (2011) J.Am. Chem. Soc., 133(6), 1662-5.)
PROTEINS

Pulse programs for PROTEIN samples (Legacy)

ARTSY-2D TROSY for measuring 1DNH RDCs on perdeuterated proteins

ARTSY-2D TROSY for measuring 1DNH RDCs on protonated proteins

ARTSY-3D TROSY for measuring 1DNH RDCs on perdeuterated proteins

3D version, 2D 13C-HSQC modulated in 3rd dimension by 1JCH (for RDCs)

1D self diffusion measurement by stimulated echo using incremented gradient pulse duration

3D HNCO 1JNH coupled in the 15N dimension (antiphase) (for RDCs)

3D HACACON for 1JCAHA (antiphase coupled - for RDCs)

3D HNCA for measuring couplings in CA dimension (e.g. 1JCAHA and RDCs)
NUCLEIC ACIDS

Pulse programs for NUCLEIC ACID samples

2D NOESY with presat

2D NOESY with 1-1 echo solvent suppression

2D NOESY with 3-9-19 watergate solvent suppression

2D NOESY with Watergate 5 solvent suppression

1H-15N 2D HMQC

1H-13C 2D HMQC

2D 1H Total Correlation

2D 1H-1H Correlation

F2 filtered 2D NOESY

F1/F2 filtered 2D NOESY

F1 filtered/F2 edited 2D NOESY

ARTSY HSQC to measure aromatic C-H RDCs

ARTSY HSQC to measure imino N-H RDCs

ARTSY HSQC to measure C5-H5 RDCs
Adjunct .incl files

Required include file for general NA Pulse Program functioning on Bruker spectrometers

Required include file for NMRFAM PR Pulse Program functioning on Bruker spectrometers
Required prosol relations
 (e.g. for Topspin 3.2.5 place these in /opt/topspin3.2.5/conf/instr/spect/prosol/pulseassign)




Useful macros for TopSpin/XWINNMR

unified scale referencing for X-nucleus indirect dimension of n-dimensional spectra


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