Sparky Output Instruction

	Native probabilistic backbone assignment output format generated by PINE
	Important Note: PINE produces a probabilistic assignment for every residue. This means that it is possible for some residues to have more than one candidate spin system for their chemical shifts. In these cases, an associated probability is assigned to each candidate spin system assigned to a residue. The sum of all probabilities always adds to one. See the sample output shown in the table below. The probability for the system is shown columns marked with P(H,N). A probability of 1 indicates an extremely confident assignment. A value of 0 columns indicates that no candidate spin system with a reasonable probability was found to match that residue. When more than one possibility is detected (see row 11), the structure in the columns 3-5 for the given residue is repeated and the alternative probabilities and associated chemical shift assignments for 1H and 15N are specified. The table shows portions of the backbone assignment with computed probabilities.

	Residue_Name P(H,N) H N CO CA CB P(H,N) H N P(H,N) H N P(H,N) H N P(no_assignment) 1 MET 0.000 0.000 0.00 0.00 55.40 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 2 GLN 0.602 7.851 120.31 0.00 55.40 0.00 0.396 8.623 123.91 0.001 8.041 123.94 0.000 0.000 0.00 0.000 3 ILE 1.000 7.851 120.31 0.00 59.63 42.06 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 4 PHE 1.000 8.593 118.52 0.00 55.11 41.23 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 5 VAL 1.000 9.301 121.32 0.00 60.51 34.23 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 6 LYS 1.000 8.868 127.82 0.00 54.57 34.46 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 7 THR 1.000 8.733 115.59 0.00 60.56 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 8 LEU 1.000 9.113 121.46 0.00 57.51 41.92 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 9 THR 1.000 7.635 105.98 0.00 61.49 69.09 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 10 GLY 1.000 7.807 109.28 0.00 45.35 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 11 LYS 1.000 7.262 121.99 0.00 56.27 33.37 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 12 THR 1.000 8.648 120.71 0.00 62.45 69.80 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 13 ILE 1.000 9.508 127.78 0.00 60.02 40.76 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 14 THR 1.000 8.743 121.88 0.00 62.12 69.67 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 15 LEU 1.000 8.717 125.16 0.00 52.74 46.90 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 16 GLU 1.000 8.121 122.53 0.00 54.91 29.66 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 17 VAL 1.000 8.924 117.59 0.00 58.40 36.34 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 18 GLU 1.000 8.640 119.33 0.00 52.71 30.83 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 19 PRO 0.000 0.000 0.00 0.00 65.31 31.88 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 20 SER 0.966 7.022 103.50 0.00 57.41 63.38 0.003 8.623 123.91 0.000 0.000 0.00 0.000 0.000 0.00 0.031 21 ASP 1.000 8.041 123.94 0.00 55.87 40.88 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 22 THR 1.000 7.875 109.08 0.00 59.68 71.19 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 23 ILE 0.928 8.303 124.28 0.00 53.84 44.35 0.072 8.526 121.36 0.000 0.000 0.00 0.000 0.000 0.00 0.000 24 GLU 0.755 9.233 118.93 0.00 55.62 31.49 0.232 8.623 123.91 0.000 0.000 0.00 0.000 0.000 0.00 0.012 25 ASN 1.000 8.303 124.28 0.00 55.40 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 26 VAL 0.995 7.851 120.31 0.00 67.65 0.00 0.005 9.233 118.93 0.000 0.000 0.00 0.000 0.000 0.00 0.000 27 LYS 1.000 8.543 119.05 0.00 59.21 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 28 ALA 0.554 7.985 123.61 0.00 55.40 0.00 0.402 8.623 123.91 0.011 8.140 123.31 0.002 9.233 118.93 0.031 29 LYS 1.000 7.851 120.31 0.00 59.75 33.33 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 30 ILE 0.997 8.270 121.42 0.00 66.06 36.82 0.003 8.156 120.44 0.000 0.000 0.00 0.000 0.000 0.00 0.000 31 GLN 1.000 8.541 123.67 0.00 60.06 27.70 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 32 ASP 1.000 8.016 119.84 0.00 57.39 40.98 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 33 LYS 1.000 7.415 115.57 0.00 58.19 33.98 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 34 GLU 1.000 8.715 114.29 0.00 55.34 33.22 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 35 GLY 1.000 8.499 108.95 0.00 45.99 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 36 ILE 1.000 6.130 120.34 0.00 57.78 40.50 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 37 PRO 0.000 0.000 0.00 0.00 0.00 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 38 PRO 0.000 0.000 0.00 0.00 66.10 32.83 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 39 ASP 1.000 8.529 113.74 0.00 55.82 39.77 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 40 GLN 1.000 7.815 117.00 0.00 55.62 30.09 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 41 GLN 1.000 7.478 118.12 0.00 56.67 31.49 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 42 ARG 1.000 8.508 123.08 0.00 55.14 31.78 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 43 LEU 1.000 8.823 124.50 0.00 53.00 45.75 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 44 ILE 1.000 9.052 122.28 0.00 58.90 41.16 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 45 PHE 1.000 8.833 125.41 0.00 56.72 43.67 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 46 ALA 1.000 8.915 132.89 0.00 52.53 16.51 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 47 GLY 1.000 8.149 102.60 0.00 45.35 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 48 LYS 1.000 7.976 122.02 0.00 54.57 34.40 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 49 GLN 1.000 8.634 122.92 0.00 55.87 29.08 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 50 LEU 0.780 8.540 125.75 0.00 53.84 44.35 0.220 8.303 124.28 0.000 0.000 0.00 0.000 0.000 0.00 0.000 51 GLU 1.000 9.233 118.93 0.00 55.93 31.84 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 52 ASP 0.906 8.156 120.44 0.00 55.10 45.18 0.089 8.315 125.39 0.002 8.540 125.75 0.002 8.368 124.49 0.000 53 GLY 0.999 8.287 115.37 0.00 45.16 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 54 ARG 1.000 7.452 119.43 0.00 54.33 32.66 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 55 THR 1.000 8.805 108.87 0.00 59.72 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 56 LEU 1.000 8.132 118.12 0.00 58.66 40.24 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 57 SER 1.000 8.470 113.59 0.00 61.20 62.55 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 58 ASP 1.000 7.928 124.60 0.00 57.36 40.28 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 59 TYR 1.000 7.242 115.84 0.00 58.27 40.01 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 60 ASN 1.000 8.142 116.09 0.00 54.13 37.34 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 61 ILE 1.000 7.239 118.96 0.00 62.46 36.67 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 62 GLN 1.000 7.621 125.03 0.00 53.59 31.59 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 63 LYS 1.000 8.490 120.65 0.00 57.84 32.56 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 64 GLU 1.000 9.301 114.57 0.00 58.30 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 65 SER 1.000 7.657 115.03 0.00 60.97 64.94 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 66 THR 1.000 8.739 117.47 0.00 62.50 70.20 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 67 LEU 1.000 9.409 127.61 0.00 53.84 44.35 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 68 HIS 0.966 9.233 118.93 0.00 55.62 31.49 0.034 9.194 127.19 0.000 0.000 0.00 0.000 0.000 0.00 0.000 69 LEU 1.000 8.303 124.28 0.00 53.84 44.35 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 70 VAL 0.910 9.233 118.93 0.00 55.62 31.49 0.066 7.851 120.31 0.022 8.623 123.91 0.000 0.000 0.00 0.000 71 LEU 0.952 8.303 124.28 0.00 53.84 44.35 0.048 8.543 119.05 0.000 0.000 0.00 0.000 0.000 0.00 0.000 72 ARG 1.000 9.233 118.93 0.00 55.10 30.59 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 73 LEU 1.000 8.287 115.37 0.00 54.95 42.37 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 74 ARG 1.000 8.449 122.06 0.00 56.20 30.92 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 75 GLY 1.000 8.518 110.48 0.00 45.24 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000 76 GLY 1.000 8.345 108.79 0.00 47.15 43.52 0.000 0.000 0.00 0.000 0.000 0.00 0.000 0.000 0.00 0.000

Native probabilistic backbone assignment output format generated by PINE