PINE Server v.2.0
Bahrami, A., Assadi, A., Markley, J. L. & Eghbalnia, H., Probabilistic Interaction Network of Evidence Algorithm and its Application to Complete Labeling of Peak lists from Protein NMR Spectroscopy, PLoS Comput Biol. 2009 Mar;5(3):e1000307.
PINE accepts, as input, the sequence of the protein plus peak lists (or spin systems) from a variety of NMR experiments and offers automatic backbone and sidechain assignments, detection and automated correction of potential referencing problems or inconsistent assignments and secondary structure determination.
- Acceptable formats for input data are
- PINE also offers assignments for proteins that have been selectively labeled or have prior partial assignments.
- Instruction for PINE output files can also be found here.
- Assignment results, including secondary structure identification, are returned via email.
- Depending on the size of the protein, the quality of input data, and number of current jobs, run times vary from one hour to one day.
- Please checkout the new Sparky extension PINE-SPARKY for
verification of PINE automated assignments. It is already pre-installed in NMRFAM-SPARKY (download link).
Lee, W., Westler, W. M., Bahrami, A., Markley, J. L., PINE-SPARKY: graphical interface for evaluating automated probabilistic peak assignments in protein NMR spectroscopy. Bioinformatics. 2009 Aug 15;25(16):2085-7. Epub 2009 Jun 3..
2) If you have already analyzed the spin systems, you can upload your spin system file in correct format in the spin system field at the end of the webpage. In that case please do NOT upload peak lists, however you still need to upload the sequence file in correct format.
A service offered by the National Magnetic Resonance Facility at Madison. Updated JULY 2014.
OPTIONAL: Prot_file, Pre-assignment, Selective_Labeling, Spin_Systems